Teacher Information
- Name
- SHAO Fangwei ZJU Faculty Homepage
- Department
- ZJUI
- Research
-
Nucleic Acid Chemistry, Bioinorganic Chemistry, Chemical Biology, and DNA Nanotechnology
- Document Type
- Book Chapter
Publication
- Chapter Title
-
Simultaneous RNA–DNA FISH
- Author
- Page
-
135-145
- Source Title
-
Long Non-Coding RNAs: Methods and Protocols
- Author/Author of Source
- Publisher
-
Springer
- P-ISBN
-
9781493933761
- E-ISBN
-
9781493933785
- Abstract
-
A highly useful tool for studying lncRNAs is simultaneous RNA–DNA FISH, which reveals the localization and quantitative information of RNA and DNA in cellular contexts. However, a simple combination of RNA FISH and DNA FISH often generates disappointing results because the fragile RNA signals are often damaged by the harsh conditions used in DNA FISH for denaturing the DNA. Here, we describe a robust and simple RNA–DNA FISH protocol, in which amino-labeled nucleic acid probes are used for RNA FISH. The method is suitable to detect single-RNA molecules simultaneously with DNA.
- DOI
- https://doi.org/10.1007/978-1-4939-3378-5_11
Print & Electronic Holdings
- Database
-
Springer
- Link